| 质粒名称: | pGEX-6p-1-hsG3BP1 D129A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000939 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | G3BP1 |
| 物种来源: | human |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-hsG3BP1 D129A载体质粒基本信息
pGEX-6p-1-hsG3BP1 D129A质粒图谱载体图谱和pGEX-6p-1-hsG3BP1 D129A载体序列质粒序列多克隆位点信息
pGEX-6p-1-hsG3BP1 D129A质粒载体简介
pGEX-6p-1-hsG3BP1 D129A质粒序列载体序列
LOCUS pGEX-6p-1-hsG3BP1 D129A 6394 bp DNA circular SYN 13-APR-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6394)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6394
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
CDS 951..971
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 978..2378
/codon_start=1
/label=hsG3BP
/translation="MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGL
DSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQA
LRRFMQTFVLAPEGSVANKFYVHNAIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTP
EVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEET
APEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQ
PRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSH
QLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRP
IMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGV
GRGLAPRQ"
misc_feature complement(1335..1373)
/label=Primer R
misc_feature 1353..1391
/label=Primer F
misc_feature 1362..1364
/label=D129A
promoter 2697..2801
/gene="bla"
/label=AmpR promoter
CDS 2802..3662
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3833..4421
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4665..4742
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4743..5825
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 5838..5859
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5874..5904
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5912..5928
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5936..5952
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5948..6394
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(5968..5984)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaacctgt
961 atttccaggg cggtagtatg gttatggaaa aaccgagtcc gctgctggtt ggtcgcgaat
1021 ttgtgcgtca gtattatacc ctgctgaatc aggccccgga tatgctgcat cgtttttatg
1081 gtaaaaatag cagttacgtg catggtggtc tggatagcaa tggtaaaccg gcagatgccg
1141 tgtatggtca gaaagaaatt catcgtaaag tgatgagtca gaattttacc aattgccata
1201 ccaaaattcg tcatgttgat gcccatgcaa ccctgaatga tggcgtggtg gtgcaggtta
1261 tgggtctgct gagtaataat aatcaggcac tgcgccgctt tatgcagacc tttgttctgg
1321 caccggaagg tagtgttgca aataagtttt atgtgcataa cgccatcttc cgttatcagg
1381 atgaagtgtt tggcggcttt gtgaccgaac cgcaggaaga aagtgaagaa gaagttgaag
1441 aaccggaaga acgtcagcag accccggaag ttgtgccgga tgatagtggt accttttatg
1501 atcaggccgt ggttagcaat gatatggaag aacatctgga agaaccggtt gcagaaccgg
1561 aaccggaccc tgaaccggaa cctgaacagg aaccggtgag cgaaattcag gaagaaaaac
1621 cggaaccggt tctggaagaa accgccccgg aagatgccca gaaaagcagt agtccggcac
1681 cggcagatat tgcccagacc gttcaggaag atctgcgcac ctttagttgg gcaagcgtta
1741 ccagcaaaaa tctgccgccg agcggtgccg ttccggtgac aggtattccg ccgcatgtgg
1801 ttaaagttcc ggccagtcag ccgcgtccgg aaagtaaacc ggaaagccag attccgccgc
1861 agcgtccgca gcgtgatcag cgtgttcgtg aacagcgtat taatattccg ccgcaacgcg
1921 gcccgcgccc gattcgtgaa gccggtgaac agggcgatat tgaaccgcgt cgtatggtgc
1981 gtcatccgga tagccatcag ctgtttattg gtaatctgcc gcatgaagtt gataaaagcg
2041 aactgaaaga tttctttcag agctatggta atgtggtgga actgcgcatt aatagtggtg
2101 gcaaactgcc gaattttggt tttgtggtgt ttgatgatag tgaaccggtt cagaaagttc
2161 tgagtaatcg cccgattatg tttcgtggcg aagttcgtct gaatgtggaa gaaaagaaaa
2221 cccgtgccgc acgtgaaggc gatcgccgcg ataatcgtct gcgtggcccg ggtggcccgc
2281 gtggtggttt aggtggtggc atgcgcggtc cgccgcgtgg tggcatggtt cagaaaccgg
2341 gttttggtgt tggtcgcggc ctggccccgc gccagtaact cgagcggccg catcgtgact
2401 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
2461 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
2521 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
2581 gcggagtgta taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
2641 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
2701 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
2761 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
2821 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
2881 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
2941 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
3001 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
3061 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
3121 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
3181 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
3241 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
3301 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
3361 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
3421 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
3481 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
3541 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
3601 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
3661 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
3721 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
3781 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
3841 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
3901 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
3961 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
4021 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
4081 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
4141 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
4201 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
4261 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
4321 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
4381 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
4441 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
4501 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
4561 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
4621 ttctccttac gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggt
4681 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
4741 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
4801 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
4861 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
4921 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
4981 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
5041 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
5101 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
5161 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
5221 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
5281 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
5341 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
5401 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
5461 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
5521 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
5581 catgttatat cccgccgtca accaccatca aacaggattt tcgcctgctg gggcaaacca
5641 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
5701 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
5761 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
5821 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
5881 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
5941 aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga
6001 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
6061 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
6121 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
6181 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
6241 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
6301 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
6361 ccagacgcga attatttttg atggcgttgg aatt
//
